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2019 iGEM team Linkoping Sweden

2019 iGEM team Linkoping Sweden validated this part.

Binding assay for CBD-sfGFP

Eppendorf tubes with varying mass of cellulose (0, 0.002, 0.04, 0.06, 0.08, 0.1 g) was mixed with a constant volume of CBD-sfGFP lysate (700 uL). The tubes was vortexed together with an Eppendorf tube containing PBS buffer as a control. The tubes was then attached to a end-to-end rotator for 30 minutes. After the end-to-end rotator the tubes was placed in a tube holder so the cellulose powder could settle in the bottom of the tubes and the solution containing lysate was clear of cellulose. When the solution was clear from cellulose, 100 µL from each of the solutions was applied on a plate reader which measured the fluorescence of CBD-sfGFP at xx nm. The results from the binding assay, see figure xx, shows that increasing the mass of cellulose powder increased the binding of CBD-sfGFP (see Figure 1.)

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